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KMID : 0356419990170030143
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Journal of Korean Andrology
1999 Volume.17 No. 3 p.143 ~ p.149
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Regulation of TGF-β1 and Collagen Expression by Antisense Oligodeoxynucleotide in Human Corpus Cavernosum Smooth Muscle Cells
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Park Kwang-Sung
Kim Hye-Joung
Lee Jeong-Eun
Lee Seung-Chul
Ryu Soo-Bang
Lee Hyun-Chul
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Abstract
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Purpose: Transforming growth factor (TGF)-β1 stimulates collagen synthesis in human corpus cavernosum smooth muscle cells (HCCSMC). We studied whether an antisense TGF-β1 oligodeoxynucleotide changed TGF-β1 mRNA expression and its related collagen synthesis.
Materials and Methods: The HCCSMC were cultured primarilly from three impotent patients. Expression of TGF-β1 mRNA was induced by phorbol 12-myristate 13-acetate (PMA) 0.2 ㎍/mL in the presence or absence of antisense TGF-β1 oligodeoxynucleotide (5’-GGGCGGCATGGGGGA-3’) 20 ㎍/mL. The effect of the antisense oligodeoxynucleotide on TGF-β1 mRNA and collagen I synthesis were measured by RT-PCR or Northern blot analysis.
Results: The expression of TGF-β1 mRNA in response to PMA was inhibited by treatment with the antisense oligodeoxynucleotide. Collagen I expression was also induced by PMA treatment, and the antisense oligodeoxynucleotide downregulated this expression.
Conclusions: Antisense TGF-β1 oligodeoxynucleotide can suppress the expression of TGF-β1 and TGF-β1-induced collagen I synthesis in HCCSMC.
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KEYWORD
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Collagen, Corpus cavernosum, Transforming growth factor beta
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